Silence of the Genes: Using Light Scattering Technologies to Measure Micro-RNA Nanoparticles Designed for Gene Silencing


  • Date
    June 8, 2017
  • Time
    8:00 a.m. PDT / 11:00 a.m. EDT / 16:00 BST / 17:00 CEST - Duration: 60 Minutes

Date : June 8, 2017
Time : 8:00 a.m. PDT / 11:00 a.m. EDT / 16:00 BST / 17:00 CEST - Duration: 60 Minutes
  • Overview

    Accurately measuring the stability of biological molecules is a critically important step in the development of novel biologic-based therapeutics. Since nanoparticle properties such as size and zeta potential may influence particle uptake by target cells and/or tissues in vivo, light scattering technologies represent an important tool to investigate the physicochemical properties of miRNA-delivery systems. Here we demonstrate that the Litesizer™ 500 returns rapid and precise results despite very low sample concentration and volume. We used dynamic light scattering (DLS) for particle size measurement and a novel, patented electrophoretic light scattering technology called cmPALS for zeta potential determination.
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  • Speakers

    Andreas Zimmer, Ph.D.,
    Professor,
    Karl-Franzens-University
    Betty Petrillo, Ph.D.,
    International Product Manager,
    Anton Paar
    Alexandra Taylor,
    Associate Editor,
    C&EN