Accurately measuring the stability of biological molecules is a critically important step in the development of novel biologic-based therapeutics. Since nanoparticle properties such as size and zeta potential may influence particle uptake by target cells and/or tissues in vivo, light scattering technologies represent an important tool to investigate the physicochemical properties of miRNA-delivery systems. Here we demonstrate that the Litesizer™ 500 returns rapid and precise results despite very low sample concentration and volume. We used dynamic light scattering (DLS) for particle size measurement and a novel, patented electrophoretic light scattering technology called cmPALS for zeta potential determination.
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Continuously Monitored Phase Analysis Light Scattering (cmPALS) enables faster and more reproducible zeta potential analysis. Specifically, the technology includes an additional monitor that measures and removes any non-linearities in the frequency modulator and thus enables faster measurements at lower voltages with improved reproducibility.