In this seminar, we explore method development approaches from simple to complex and how different variables influence selectivity for the chromatographer. We focus in detail on stationary phase choices, gradient time, type of organic modifier and eluent pH. With an understanding of selectivity and using a variety of chromatographic data, we then discuss rational stationary phase design. This includes exploring the chromatographic selectivity and properties of uniquely designed stationary phases to understand how the weighting of different modes of interaction between the analyte and the stationary phase (hydrophobicity, aromatic interactions, dipole-dipole interactions, polar retention, phenolic resolution) effects retention and selectivity.
