With the increasing emphasis on biotherapeutics in the pharmaceutical industry, many researchers have goals of obtaining sharp, well-resolved peaks for their LC analyses of larger, complex biomolecules, such as monoclonal antibodies (mAbs), biosimilars, and fusion proteins. In this webinar, we will describe several methods to improve biomolecule separations using columns packed with Fused-Core particles. The Fused-Core particle design, with its solid silica core and thin porous shell, enables fast, rugged separations for biomolecules of various sizes, including peptides, proteins, as well as large monoclonal antibodies, and their fragments and variants.

Careful selection and adjustment of parameters, such as optimal pore size, bonded phase, gradient conditions, mobile phase modifier and additive, and column temperature, all contribute to significantly improved analysis of biotherapeutics. The importance of each of these parameters will be discussed, along with pertinent examples. In addition, to highlight the advantages of Fused-Core columns, we will compare separations using Fused-Core columns with those obtained using both totally porous particle columns and other superficially porous particle columns.