Capillary flow LC-MS combines the advantages of high-throughput analytical flow LC separations and the high sensitivity of nano-flow LC-MS analysis. This is a robust solution that can be used for routine proteomics experiments and analysis of large sample cohorts.
Recent LC and MS technological advances bring a new level of performance required to answer complex biological questions. Capillary flow LC-MS has been shown to be a valuable tool for targeted PRM peptide quantification as well as bottom-up DDA and DIA proteomics.
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Key Learning Objectives
Learn best practice guidelines for setting up and running capillary flow LC-MS methods
Recognize how LC-MS sensitivity depends on column internal diameter and flow rate
Understand which capillary flow LC-MS solution can be used for high-throughput shotgun and targeted proteomics analysis of cell lysates and biofluids
Hear which Thermo Scientific capillary-flow LC-MS solution components ensure robustness, sensitivity and throughput