UPLC-ESI-TOF MS based Metabolite Profiling of the Antioxidative
Food Supplement Garcinia buchananii


  • Date
    December 9, 2015
  • Time
    8:00 a.m. PST / 11:00 a.m. EST / 16:00 GMT / 17:00 CET - Duration: 60 Minutes 8:00 a.m. PST / 11:00 a.m. EST / 16:00 GMT / 17:00 CET - Duration: 60 Minutes

Date: December 9, 2015
Time: 8:00 a.m. PST / 11:00 a.m. EST / 16:00 GMT / 17:00 CET - Duration: 60 Minutes
  • Overview

    In this webinar we review this analyses and introduce Progenesis QI software and illustrate its use for the quantification and identification of compounds in the different preparations of the antioxidant food supplement Garcinia buchananii. Comparative antioxidative analyses of aqueous ethanolic extracts from leaf, root, and stem of Garcinia buchananii revealed high activity of all three organs. To investigate the metabolites composition of the different parts of G. buchananii, an untargeted metabolomics approach using UPLC-ESI-TOF MS with simultaneous acquisition of low- and high-collision energy mass spectra (MSe) was performed. Unsupervised statistics (PCA) highlighted clear differences in the metabolomes of the three organs. OPLS-DA revealed (2R,3S,2''R,3''R)-GB-1, (2R,3S)-morelloflavone, and (2R,3S)-volkensiflavone as the most decisive marker compounds discriminating leaf from root and stem extract. Read more
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  • Speakers

    Dr Timo Stark, Ph. D
    Senior Scientist and Head of LC-MS at the Chair of Food Chemistry and Molecular Sensory Science,
    Technical University of Munich (TUM)
    Dr Robert Tonge, Ph. D
    Principal Product Manager, OMICS Informatics,
    Waters Corporation
    Matt Davenport
    Associate Editor,
    C&EN