In this webinar we review this analyses and introduce Progenesis QI software and illustrate its use for the quantification and identification of compounds in the different preparations of the antioxidant food supplement
Garcinia buchananii. Comparative antioxidative analyses of aqueous ethanolic extracts from leaf, root, and stem of
Garcinia buchananii revealed high activity of all three organs. To investigate the metabolites composition of the different parts of
G. buchananii, an untargeted metabolomics approach using UPLC-ESI-TOF MS with simultaneous acquisition of low- and high-collision energy mass spectra (MS
e) was performed. Unsupervised statistics (PCA) highlighted clear differences in the metabolomes of the three organs. OPLS-DA revealed (2R,3S,2''R,3''R)-GB-1, (2R,3S)-morelloflavone, and (2R,3S)-volkensiflavone as the most decisive marker compounds discriminating leaf from root and stem extract.
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Leaves represent the best source to isolate GB-1, morelloflavone, and volkensiflavone. Root extract is the best organ to isolate xanthones, stem bark extract the best source to isolate (2R,3S,2''R,3''R)-manniflavanone, and the identified polyisoprenylated benzophenones are characteristic compounds for the leaf organ. Morelloflavone, volkensiflavone and garcicowin C were isolated for the first time from G. buchananii and identified via MS, NMR, and CD spectroscopy and showed in H2O2 scavenging, H/L-TEAC, and H/L-ORAC assays moderate to strong in vitro antioxidative activities.
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