C&EN App Note
Automated Switching Between Peptide and Glycan Mapping
Brought to you by Agilent Technologies

Glycan and peptide mapping are key analytical methodologies for the structural characterization of therapeutic proteins such as monoclonal antibodies (mAbs). This application note describes glycan and peptide mapping of mAbs performed on the same system without manual intervention upon switching between modes. The instrumental setup offers great flexibility and productivity for performing glycan and peptide mapping in one single analytical sequence. Highly informative and precise data is obtained in the analysis of originator and biosimilar mAbs.

Key Objectives:
  • How to achieve rapid glycan release and labeling
  • How to switch between glycan and peptide mapping without manual intervention
  • How to switch between glycan and peptide mapping without manual intervention

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Agilent Technologies
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