Isolation of Green Tea Catechins Using Preparative HPLC

Isolation of Green Tea Catechins Using Preparative HPLC

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Overview

Tea is not only one of the most widely consumed beverages in the worldâ€”its high antioxidant content also makes it the beverage most believed to have health-promoting effects.

This application demonstrates the isolation of catechins, the most important group of antioxidants in green tea. Despite the complex composition of green tea, five catechins collected had purity of 95% or higher.

Key Objectives:

Separate green tea extract by reversed-phase LC/MS
Use a fraction collector to isolate single compounds
Trigger fraction collection by MS signal for highest selectivity
Reanalyze fractions to assess purity and gain basic structural information

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