C&EN App Note
Isolation of Green Tea Catechins Using Preparative HPLC
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Tea is not only one of the most widely consumed beverages in the world—its high antioxidant content also makes it the beverage most believed to have health-promoting effects.

This application demonstrates the isolation of catechins, the most important group of antioxidants in green tea. Despite the complex composition of green tea, five catechins collected had purity of 95% or higher.

Key Objectives:
  • Separate green tea extract by reversed-phase LC/MS
  • Use a fraction collector to isolate single compounds
  • Trigger fraction collection by MS signal for highest selectivity
  • Reanalyze fractions to assess purity and gain basic structural information

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Agilent Technologies
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