C&EN App Note
Automated Monoclonal Antibody Subunit Analysis by Online Reduction Using 2D-LC/MS
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Recombinant monoclonal antibodies (mAbs) have become a popular class of biotherapeutics over recent years. Subunit analysis is a possible strategy to assess critical quality attributes in mAb products.

This application note describes a fully automated workflow for the purification, online reduction, and mass spectrometry (MS) analysis of mAb subunits using two-dimensional liquid chromatography (2D-LC).

Key Objectives:
  • Reducing disulfide bonds divides mAbs into heavy and light chain subunits
  • 2D-LC can be operated with a trap column, allowing online reduction
  • MS-compatible SEC can desalt and separate mAb subunits

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Agilent Technologies
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