C&EN eBook
Chromapedia – Complete guide of essential knowledge for developing and performing optimal flash chromatography and preparative HPLC separations
Brought to you by Buchi

This guide offers everything you need to know to accelerate your purification workflow with automated flash and preparative HPLC. You’ll find concise theory, plus practical insights and tips for method development and optimization. Dig in deeper with topics like advanced method development, scale-up, and solutions for complex samples.
Key Objectives:
  • Determine the best detection method for your application
  • Select the ideal column or cartridge, plus how to use and maintain consumables
  • Choose the ideal stationary phase and mobile phase
  • Identify method parameters using TLC and analytical chromatography before scale-up to flash or prep HPLC
  • Improve resolution using principles of efficiency, retention, and selectivity

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