Creating Better Chromatographic Methods Using a Systematic Approach

DATE

February 21, 2023

TIME

8:00 a.m. PST / 11:00 a.m. EST / 16:00 GMT / 17:00 CET - Duration: 60 Minutes

Overview

In this presentation, we will discuss a systematic approach to LC method development, including an overview on how to ensure your LC system is performing optimally as well as the three main mechanisms that are available to adjust the selectivity of a separation:

Column chemistry
Organic solvent
Mobile phase pH

We’ll illustrate these mechanisms using a test mix of acids, bases and neutrals. We’ll also discuss the impact column hardware plays in methods development. Utilizing this knowledge, we will demonstrate this approach using a real-world example, the forced degradation of deferoxamine.

Key Learning Objectives:

Learn how column chemistry, organic solvent and mobile phase pH influence the selectivity of chromatographic separations
Learn about a systematic method development approach used to develop a separation
Learn how to choose the “best” scouting separation for each sample and the steps taken to optimize that separation
Learn about the latest 2.5 µm column technologies and how they can enhance your methods and results

Who Should Attend:

Method Development Scientists
QA/QC Scientists
Laboratory Managers & Directors

Brought to you by:

Speakers

Kenneth Berthelette
Sr Scientist: Consumables and Lab Automation Group,
Waters Corporation

Catherine Dold
Health & Environment Writer,
C&EN Media Group

Registration

Forwarded this event? Clear the form

Email Address*

Salutation

First Name*

Last Name*

Company*

Job Title*

Address 1*

City*

State*

Zip / Post Code*

Country*

Business Phone*

What are the primary challenges that you encounter when developing methods?*

Do you experience challenges with reproducible results when working with metal sensitive analytes?*

How often do you work with work with metal sensitive analytes?*

I would like to receive more information on products and services from the American Chemical Society

*By submitting this form, you agree to receive more information on related products and services from the American Chemical Society (ACS Publications) and its sponsor via email. ACS takes your privacy seriously. For more information, please see the ACS Privacy Policy.

View On Demand Webinars