Comprehensive characterization of complex macromolecular structures is challenging since no single analytical technique provides adequate information regarding all the different distributions. Hyphenated techniques, that couple separation by asymmetric-flow field-flow fractionation (AF4) or size-exclusion chromatography (SEC) with a multi-detection system, do provide detailed insight into such structures. A versatile online multi-detection system includes multi-angle light-scattering (MALS), dynamic light scattering (DLS), ultraviolet (UV) and refractive-index (RI) detectors.
This webinar explores the application of SEC-MALS-DLS-UV-RI and AF4-MALS-DLS-UV-RI to understanding the molar mass, composition, size, distributions and structure of hybrid polymers and supramolecular structures. Examples include star copolymers, protein-poly(ethylene glycol) conjugates, large unilamellar vesicles, lipid droplets and exosomes.