High Throughput Analysis for Therapeutic Protein Development

June 13, 2023
8:00 a.m. PDT / 11:00 a.m. EDT / 16:00 BST / 17:00 CEST - Duration: 60 Minutes


When developing proteins as biopharmaceuticals, understanding structural stability is essential in product and process development. However, available material, time, and resources is essential in product and process development. However, available material, time and resources limit biophysical analysis to a few key measurements.

Dynamic light scattering (DLS) which characterizes size and aggregation of macromolecules or nanoparticles, is an industry-standard technique for protein formulation and stability evaluation. This webinar will illuminate how high-throughput DLS (HT-DLS), performed in situ in low-volume microwell plates, is used to understand aggregation, colloidal and thermal stability of therapeutic protein candidates and formulations. In HT-DLS, dozens to hundreds of samples can be characterized by a single plate scan, or by multiple scans across a temperature ramp. Application examples include freeze-thaw studies, formulation buffer screening and thermal stability analysis.

Key Learning Objectives:
  • Background and instrumentation for HT-DLS
  • How DLS is used to study aggregation under applied stresses
  • How DLS evaluates protein colloidal stability via second virial coefficient A2 and the diffusion interaction parameter kD.
Who Should Attend:
  • Biopharma product development scientists in need of automated methods for characterizing protein stability
  • Formulation lab managers seeking to improve productivity with high-throughput DLS instrumentation

Brought to you by:
Wyatt Technology


Felix Gloge, Ph.D.
Field Application Scientist,
Wyatt Technology
Christian Sieg, Ph.D.
Application Scientist,
Wyatt Technology
Melissa O'Meara
Forensic Science Consultant,
C&EN Media Group


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