This webinar will present the use of dynamic light scattering (DLS), electrophoretic light scattering (ELS), and various microscopy methods to understand pH and pH- and temperature-triggered swelling transitions in a unique class of “ultra-low-cross-linked” hydrogel microparticles. An introduction to dynamic light scattering and phase-analysis light scattering will be provided as well.

The complex interplay between thermally-driven polymer phase separation, charge segregation, and nanostructure evolution present a set of particularly difficult characterization challenges that are overcome by employing a suite of complementary yet orthogonal analytical tools. Given the potential utility of this class of materials for a wide range of applications in biotechnology, obtaining a deep level of insight into their properties is of paramount importance when considering efficacy, safety, and overall materials performance.