High pressure liquid chromatography (HPLC) is a benchmark technique used in laboratories across the world. However, even experienced analysts that are familiar with HPLC concepts and principles can fall victim to chromatographic pitfalls that arise. Some common issues include misshapen peaks, unstable baselines, problems with reproducibility,and the observance of irregular back pressure. Identifying and correcting these problems quickly can not only save time but increase productivity in the lab. Chromatographic issues can arise from any part of the system, including the pump, autosampler, column oven, or detector, and each component of the system must be considered individually.