Today’s chromatographers face the same challenges that chromatographers faced 25years ago—to develop methods that maximize resolution between their analytes of interest. Selection of an appropriate pH for the analytes of interest is critical for ionizable compounds. Once the stationary phase, organic solvent, and pH are selected, vary temperature and gradient time. Then use of a commercially available method optimization software package is recommended. While C18 is a good all-purpose phase for initial method development, the other phases offer alternate selectivities. It is good practice to screen multiple phases to ensure the best possible separation.
- Learn how to utilize unique stationary phase chemistries to maximize selectivity in HPLC/UHPLC and LC/MS
- Learn about new stationary phase technology that will improve analyte resolution and lab productivity
- Review examples of using different bonded phases to improve LC and LC-MS separations