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Characterizing Vaccines with the Light Scattering Toolkit
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This paper includes a brief introduction to light scattering (LS) instruments and techniques, and how they are coupled with fractionation techniques. For several classes of vaccines – including subunit vaccines, mRNA-LNPs, polysaccharide conjugates, and plain old viruses – case studies demonstrate the utility and breadth of analyses using the light scattering toolkit.

These include measuring size distributions, characterizing protein-protein binding, and quantifying glycan content in glycoproteins or nucleic acid cargo in nanocarriers. Multiple ways to detect aggregation and other measures of stability are also described.

Key Objectives:
  • The basic types of analytical LS and the information they provide
  • How LS may be used to evaluate vaccine stability and particle concentration
  • The uses of LS in developing and manufacturing viral and non-viral gene vector vaccines
  • The benefits of LS for characterizing glycosylation and viral glycoprotein-antibody binding

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