This paper includes a brief introduction to light scattering (LS) instruments and techniques, and how they are coupled with fractionation techniques. For several classes of vaccines â€“ including subunit vaccines, mRNA-LNPs, polysaccharide conjugates, and plain old viruses â€“ case studies demonstrate the utility and breadth of analyses using the light scattering toolkit.
These include measuring size distributions, characterizing protein-protein binding, and quantifying glycan content in glycoproteins or nucleic acid cargo in nanocarriers. Multiple ways to detect aggregation and other measures of stability are also described.
- The basic types of analytical LS and the information they provide
- How LS may be used to evaluate vaccine stability and particle concentration
- The uses of LS in developing and manufacturing viral and non-viral gene vector vaccines
- The benefits of LS for characterizing glycosylation and viral glycoprotein-antibody binding